Whole genome sequencing of C. lanienae strain RM8966 - SRA

SRX2188304: Whole genome sequencing of C. lanienae strain RM8966
1 ILLUMINA (Illumina MiSeq) run: 898,004 spots, 218.7M bases, 122.7Mb downloads

Design: Illumina libraries were prepared for all strains using the KAPA Low-Throughput Library Preparation Kit with Standard PCR Amplification Module (Kapa Biosystems, Wilmington, MA), following manufacturer's instructions except for the following changes: 750 ng DNA was sheared at 30 psi for 40 s and size selected to 700-770 bp following Illumina protocols. Standard desalting TruSeq LT and PCR Primers were ordered from Integrated DNA Technologies (Coralville, IA) and used at 0.375 and 0.5 ľM final concentrations, respectively. PCR was reduced to 3-5 cycles. Libraries were quantified using the KAPA Library Quantification Kit (Kapa), except with 10 ľl volume and 90-s annealing/extension PCR, then pooled and normalized to 4 nM. Pooled libraries were requantified by ddPCR on a QX200 system (Bio-Rad), using the Illumina TruSeq ddPCR Library Quantification Kit and following manufacturer's protocols, except with an extended 2-min annealing/extension time. The libraries were sequenced 2 × 250 bp paired end v2 on a MiSeq instrument (Illumina) at 13.5 pM, following manufacturer's protocols.

Submitted by: USDA, ARS, WRRC

Study: Campylobacter vicugnae strain:RM8966 Genome sequencing and assembly

PRJNA341053 • SRP090374 • All experiments • All runs

show Abstracthide Abstract

Genomic and taxonomic analysis of Campylobacter lanienae and C. lanienae related organisms

Sample:

SAMN05687073 • SRS1711099 • All experiments • All runs

Organism: Campylobacter vicugnae

Library:

Name: RM8966WGS_MiSeq

Instrument: Illumina MiSeq

Strategy: WGS